Two-laser dual-immunofluorescence confocal laser scanning microscopy using Cy2- and Cy5-conjugated secondary antibodies: unequivocal detection of co-localization of neuronal markers.

@article{Wouterlood1998TwolaserDC,
  title={Two-laser dual-immunofluorescence confocal laser scanning microscopy using Cy2- and Cy5-conjugated secondary antibodies: unequivocal detection of co-localization of neuronal markers.},
  author={Floris G Wouterlood and J. Christiaan van Denderen and Nico Blijleven and Jan van Minnen and Wolfgang H{\"a}rtig},
  journal={Brain research. Brain research protocols},
  year={1998},
  volume={2 2},
  pages={149-59}
}
The ability of the confocal laser scanning microscope (CLSM) to visualize in one focal plane the fluorescence associated with multiple markers renders this instrument extremely valuable for the study of co-localization of various markers in the somata and cellular processes of neurons. In the present protocol we deal with the question whether or not co-localization exists in neurons of two different neuronal markers. The conventionally used method towards answering this type of question is… CONTINUE READING

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