Two interacting mutations causing temperature-sensitive phosphatidylglycerol synthesis in Escherichia coli membranes.

@article{Nishijima1981TwoIM,
  title={Two interacting mutations causing temperature-sensitive phosphatidylglycerol synthesis in Escherichia coli membranes.},
  author={Masahiro Nishijima and Christine E. Bulawa and Christian R. H. Raetz},
  journal={Journal of bacteriology},
  year={1981},
  volume={145 1},
  pages={113-21}
}
A conditionally lethal mutant of Escherichia coli lacking phosphatidylglycerol in vivo at 42 degrees C has been previously isolated by two-stage mutagenesis (M. Nishijima and C. R. H. Raetz, J. Biol. Chem. 254:7837-7844, 1979). In the first step (designated pgsA444) the phosphatidylglycerophosphate synthetase is partially inactivated, but the resulting strain continues to make about two-thirds of the normal level of phosphatidylglycerol and is not temperature sensitive. The second lesion… CONTINUE READING

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