Two helices plus a linker: a small model substrate for eukaryotic RNase P.

@article{Carrara1995TwoHP,
  title={Two helices plus a linker: a small model substrate for eukaryotic RNase P.},
  author={Giuliano Carrara and Paolo Calandra and Paolo Fruscoloni and Glauco P. Tocchini-Valentini},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 7},
  pages={2627-31}
}
Using precursor tRNA molecules to study RNA-protein interactions, we have identified an RNA motif recognized by eukaryotic RNase P (EC 3.1.26.5). Analysis of circularly permuted precursors indicates that interruptions in the sugar-phosphate backbone are not tolerated in the acceptor stem, in the T stem-loop, or between residues A-9 and G-10. Prokaryotic RNase P will function with a minihelix consisting of the acceptor stem connected directly to the T stem-loop. Eukaryotic RNase P cannot use… CONTINUE READING

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