Two gonadotropin-releasing hormones from African catfish (Clarias gariepinus).

  title={Two gonadotropin-releasing hormones from African catfish (Clarias gariepinus).},
  author={Jan Bogerd and Ka Wan Li and Coby Janssen-Dommerholt and Henk J. Th. Goos},
  journal={Biochemical and biophysical research communications},
  volume={187 1},
Two forms of gonadotropin-releasing hormone (GnRH) have been purified from brain extracts of the African catfish, Clarias gariepinus, using reverse-phase high performance liquid chromatography (HPLC) and radioimmunoassay (RIA). The amino acid sequences of both forms of African catfish GnRH were determined using Edman degradation after digestion with pyroglutamyl aminopeptidase. In addition, both GnRHs were studied by mass spectrometry. The primary structure of African catfish GnRH I is… CONTINUE READING


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