Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis by Streptomyces antibioticus

@article{Quirs1998TwoGA,
  title={Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis by Streptomyces antibioticus},
  author={Luis M Quir{\'o}s and Ignacio Aguirrezabalaga and Carlos Olano and Carmen M{\'e}ndez and Jos{\'e} A Salas},
  journal={Molecular Microbiology},
  year={1998},
  volume={28}
}
A 5.2 kb region from the oleandomycin gene cluster in Streptomyces antibioticus located between the oleandomycin polyketide synthase gene and sugar biosynthetic genes was cloned. Sequence analysis revealed the presence of three open reading frames (designated oleI, oleN2 and oleR). The oleI gene product resembled glycosyltransferases involved in macrolide inactivation including the oleD product, a previously described glycosyltransferase from S. antibioticus. The oleN2 gene product showed… Expand
Cloning, characterization and heterologous expression of a polyketide synthase and P-450 oxidase involved in the biosynthesis of the antibiotic oleandomycin.
TLDR
The heterologous expression system reported here provides a useful tool for studying this important macrolide antibiotic and indicates that OleP is involved in the epoxidation pathway of oleandomycin biosynthesis. Expand
Functional Analysis of OleY l-Oleandrosyl 3-O-Methyltransferase of the Oleandomycin Biosynthetic Pathway in Streptomyces antibioticus
TLDR
Oleandomycin, a macrolide antibiotic produced by Streptomyces antibioticus, contains two sugars attached to the aglycon: L-oleandrose and D-desosamine, which represents an alternative route for L-olesandrose biosynthesis from that in the avermectin producer StrePTomyces avermitilis. Expand
Identification and Expression of Genes Involved in Biosynthesis of l-Oleandrose and Its Intermediatel-Olivose in the Oleandomycin ProducerStreptomyces antibioticus
ABSTRACT A 9.8-kb DNA region from the oleandomycin gene cluster inStreptomyces antibioticus was cloned. Sequence analysis revealed the presence of 8 open reading frames encoding different enzymeExpand
Interspecies complementation in Saccharopolyspora erythraea : elucidation of the function of oleP1, oleG1 and oleG2 from the oleandomycin biosynthetic gene cluster of Streptomyces antibioticus and generation of new erythromycin derivatives
TLDR
Interspecies complementation has been carried out, using two mutant strains of Saccharopolyspora erythraea to identify which of these two glycosyltransferases encodes the desosaminyltransferase and which the oleandrosyltransferase, and to demonstrate that the function of OleP1 is identical to that of EryCII in the biosynthesis of dTDP‐d‐desosamine. Expand
Identification of a sugar flexible glycosyltransferase from Streptomyces olivaceus, the producer of the antitumor polyketide elloramycin.
TLDR
The ElmGT glycosyltransferase from S. olivaceus Tü2353 can transfer different sugars into the aglycon 8-DMTC and can represent an important tool for combinatorial biosynthesis. Expand
Glycosylation Steps during Spiramycin Biosynthesis in Streptomyces ambofaciens: Involvement of Three Glycosyltransferases and Their Interplay with Two Auxiliary Proteins
TLDR
Analysis of the biosynthetic intermediates accumulated by mutant strains devoid of each of the auxiliary proteins, together with complementation experiments, revealed the interplay of glycosyltransferases with the Auxiliary proteins. Expand
Identification of Two Genes from Streptomyces argillaceus Encoding Glycosyltransferases Involved in Transfer of a Disaccharide during Biosynthesis of the Antitumor Drug Mithramycin
TLDR
It is proposed that the glycosyltransferases encoded by mtmGI and mtmGII are responsible for forming and transferring the disaccharide during mithramycin biosynthesis. Expand
Engineering deoxysugar biosynthetic pathways from antibiotic-producing microorganisms. A tool to produce novel glycosylated bioactive compounds.
A plasmid (pLN2) was generated in which genes involved in the biosynthesis of L-oleandrose in the oleandomycin producer Streptomyces antibioticus ATCC11891 were cloned. pLN2 was used to direct theExpand
Glycosylation of Macrolide Antibiotics
The oleD gene has been identified in the oleandomycin producer Streptomyces antibioticus and it codes a macrolide glycosyltransferase that is able to transfer a glucose moiety from UDP-glucoseExpand
Characterization of two glycosyltransferases involved in early glycosylation steps during biosynthesis of the antitumor polyketide mithramycin by Streptomyces argillaceus
TLDR
It is demonstrated that the glycosyltransferases MtmGIV and mtmGIII catalyze the first two glycosolation steps in mithramycin biosynthesis. Expand
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It is proposed that these three genes could participate in the intracellular glycosylation of oleandomycin and its secretion during antibiotic production. Expand
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Functional analysis of the oleB gene showed that either the first or the second half of the gene containing only one ATP‐binding domain was sufficient to confer resistance to oleandomycin, and it was shown that a Streptomyces albus strain, containing both a glycosyltransferase and the OleB protein, was capable ofglycosylating ole fandomycin and secreting the inactive Glycosylated molecule. Expand
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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