Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis by Streptomyces antibioticus

@article{Quirs1998TwoGA,
  title={Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis by Streptomyces antibioticus},
  author={Luis M. Quir{\'o}s and Ignacio Aguirrezabalaga and Carlos Olano and Carmen M{\'e}ndez and Jos{\'e} A Salas},
  journal={Molecular Microbiology},
  year={1998},
  volume={28}
}
A 5.2 kb region from the oleandomycin gene cluster in Streptomyces antibioticus located between the oleandomycin polyketide synthase gene and sugar biosynthetic genes was cloned. Sequence analysis revealed the presence of three open reading frames (designated oleI, oleN2 and oleR). The oleI gene product resembled glycosyltransferases involved in macrolide inactivation including the oleD product, a previously described glycosyltransferase from S. antibioticus. The oleN2 gene product showed… 

Identification and Expression of Genes Involved in Biosynthesis of L-Oleandrose and Its Intermediate L-Olivose in the Oleandomycin Producer Streptomyces antibioticus

TLDR
A 9.8-kb DNA region from the oleandomycin gene cluster in Streptomyces antibioticus was cloned and it was revealed that pOLV and pOLE encode all enzyme activities required for the biosynthesis of these two 2,6dideoxysugars.

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TLDR
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TLDR
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TLDR
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TLDR
Analysis of the biosynthetic intermediates accumulated by mutant strains devoid of each of the auxiliary proteins, together with complementation experiments, revealed the interplay of glycosyltransferases with the Auxiliary proteins.

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TLDR
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Glycosylation of Macrolide Antibiotics

The oleD gene has been identified in the oleandomycin producer Streptomyces antibioticus and it codes a macrolide glycosyltransferase that is able to transfer a glucose moiety from UDP-glucose

Characterization of two glycosyltransferases involved in early glycosylation steps during biosynthesis of the antitumor polyketide mithramycin by Streptomyces argillaceus

TLDR
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...

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TLDR
Functional analysis of the oleB gene showed that either the first or the second half of the gene containing only one ATP‐binding domain was sufficient to confer resistance to oleandomycin, and it was shown that a Streptomyces albus strain, containing both a glycosyltransferase and the OleB protein, was capable ofglycosylating ole fandomycin and secreting the inactive Glycosylated molecule.

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TLDR
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TLDR
Interestingly, the culture supernatant contains another enzyme activity capable of reactivating the glycosylated oleandomycin and regenerating the biological activity through the release of a glucose molecule, and it is proposed that these two enzyme activities could be an integral part of the ole fandomycin biosynthetic pathway.

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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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