Two essential components of the Saccharomyces cerevisiae transcription factor TFIIIB: transcription and DNA-binding properties.

@article{Kassavetis1991TwoEC,
  title={Two essential components of the Saccharomyces cerevisiae transcription factor TFIIIB: transcription and DNA-binding properties.},
  author={George A. Kassavetis and Blaine Bartholomew and Justin A. Blanco and Thomas Johnson and E. Peter Geiduschek},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 16},
  pages={7308-12}
}
RNA polymerase III transcription factor TFIIIB from Saccharomyces cerevisiae contains at least two polypeptides, with apparent masses of 90 and 70 kDa, that were previously identified by photocrosslinking to DNA. It is shown here that TFIIIB can be chromatographically separated into two components, each of which is required for efficient tRNA gene transcription. DNA-protein photocrosslinking experiments show these two components separately contain the 90- and 70-kDa TFIIIB-specific polypeptides… CONTINUE READING

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