Two distinct nuclear transcription factors recognize loop and bulge residues of the HIV-1 TAR RNA hairpin.

@article{Sheline1991TwoDN,
  title={Two distinct nuclear transcription factors recognize loop and bulge residues of the HIV-1 TAR RNA hairpin.},
  author={C T Sheline and L H Milocco and Katherine A Jones},
  journal={Genes & development},
  year={1991},
  volume={5 12B},
  pages={2508-20}
}
Transcriptional activation by the HIV-1 Tat protein requires specific residues in the hexanucleotide loop and trinucleotide bulge of the TAR RNA stem-loop structure found in the 5'-untranslated leader of all viral transcripts. Tat directly contacts residue U22 in the bulge and is thought to act in concert with cellular factors bound to the loop. We find that HeLa nuclear extracts contain two specific TAR RNA-binding proteins, designated TRP-1 and TRP-2, which compete for binding to the upper… CONTINUE READING
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