Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP.

@article{McGarry2004TwoDB,
  title={Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP.},
  author={K. C. McGarry and V. Ryan and J. Grimwade and A. Leonard},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 9},
  pages={
          2811-6
        }
}
  • K. C. McGarry, V. Ryan, +1 author A. Leonard
  • Published 2004
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • Initiation of DNA replication in eukaryotes, archea, and eubacteria requires interaction of structurally conserved ATP-binding initiator proteins and origin DNA to mediate assembly of replisomes. However, the specific requirement for ATP in the early steps of initiation remains unclear. This is true even for the well studied Escherichia coli replication origin, oriC, where the ATP form of initiator DnaA is necessary and sufficient for initial DNA strand separation, but the five DnaA-binding… CONTINUE READING

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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 37 REFERENCES
    Molecular Cloning: A Laboratory Manual
    • 179,734
    • PDF
    DNA replication in eukaryotic cells.
    • 2,012
    • PDF