Two-dimensional polyacrylamide gel electrophoresis of proteins.

  title={Two-dimensional polyacrylamide gel electrophoresis of proteins.},
  author={Jeffrey W Pollard},
  journal={Methods in molecular biology},
Since O'Farrell (1) introduced the improved technique for high resolution two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), it has become one of the most powerful tools for the separation and quantification of proteins from complex mixtures. The principal reason for this is that the method employs separation of denatured proteins according to two different parameters, molecular weight and isoelectric point. Consequently, it has sufficient resolution to separate individual proteins… CONTINUE READING