Two-dimensional map of the proteome of Haemophilus influenzae.

  title={Two-dimensional map of the proteome of Haemophilus influenzae.},
  author={H. Hans Langen and Bal{\'a}zs Tak{\'a}cs and Stefan Evers and Peter Berndt and Herr W. Lahm and Beat Wipf and Christopher Gray and Michael Fountoulakis},
  volume={21 2},
We have constructed a two-dimensional database of the proteome of Haemophilus influenzae, a bacterium of medical interest of which the complete genome, comprising about 1742 open reading frames, has been sequenced. The soluble protein fraction of the microorganism was analyzed by two-dimensional electrophoresis, using immobilized pH gradient strips of various pH regions, gels with different acrylamide concentrations and buffers with different trailing ions. In order to visualize low-copy-number… CONTINUE READING
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