Two-dimensional liquid chromatographic methods to examine phenylboronate interactions with recombinant antibodies.

@article{Zhang2009TwodimensionalLC,
  title={Two-dimensional liquid chromatographic methods to examine phenylboronate interactions with recombinant antibodies.},
  author={Boyan Zhang and Sean Mathewson and Huifen Chen},
  journal={Journal of chromatography. A},
  year={2009},
  volume={1216 30},
  pages={5676-86}
}
A two-dimensional liquid chromatography (2D-LC) system incorporating Agilent 1100 components was set up and 2D-LC methods were developed to determine how recombinant antibodies (rAbs) interact with a commercial boronate column. Analyses of ten rAbs demonstrated that a simple boronate affinity chromatography method cannot be generalized to separate or detect the glycation level for all of rAbs as the aggregates were also retained on the boronate column. A quantitative boronate affinity… CONTINUE READING