Two-dimensional gel electrophoretic method for mapping DNA replicons.

  title={Two-dimensional gel electrophoretic method for mapping DNA replicons.},
  author={Kevin A Nawotka and Joel A. Huberman},
  journal={Molecular and cellular biology},
  volume={8 4},
We describe in detail a method which allows determination of the directions of replication fork movement through segments of DNA for which cloned probes are available. The method uses two-dimensional neutral-alkaline agarose gel electrophoresis followed by hybridization with short probe sequences. The nascent strands of replicating molecules form an arc separated from parental and nonreplicating strands. The closer a probe is to its replication origin or to the origin-proximal end of its… CONTINUE READING

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