Two-dimensional gel electrophoresis using immobilized pH gradient tube gels.

@article{Hirano2000TwodimensionalGE,
  title={Two-dimensional gel electrophoresis using immobilized pH gradient tube gels.},
  author={Hisashi Hirano and Hiroshi Kawasaki and Hidenori Sassa},
  journal={Electrophoresis},
  year={2000},
  volume={21 2},
  pages={440-5}
}
An apparatus for the preparation of gels for immobilized pH gradient isoelectric focusing (IPG) in glass tubes was developed. Using this apparatus, the highly reproducible immobilized pH gradient can be formed with Immobilines in polyacrylamide gels, and IPG gels at all possible pH ranges can be easily prepared at low cost. The IPG tube gels in the first dimension in two-dimensional gel electrophoresis was used to separate and identify a number of rice embryo proteins in the proteome analysis… CONTINUE READING

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