Two-dimensional gel electrophoresis as analytical tool for identifying Candida albicans immunogenic proteins.

@article{Pitarch1999TwodimensionalGE,
  title={Two-dimensional gel electrophoresis as analytical tool for identifying Candida albicans immunogenic proteins.},
  author={Aida Pitarch and Mercedes Pardo and Antonio Jim{\'e}nez and Jesus Pla and Concha Gil and Miguel A. Sanchez and C{\'e}sar Nombela},
  journal={Electrophoresis},
  year={1999},
  volume={20 4-5},
  pages={1001-10}
}
This paper reports the usefulness of two-dimensional gel electrophoresis followed by Western blotting with sera from patients with systemic candidiasis in the identification of the major Candida albicans antigens. In order to have different patterns of protein expression and subcellular localization, three types of protein preparations were obtained: cytoplasmic extracts, protoplast lysates and proteins secreted by protoplasts regenerating their cell wall. These proteins were separated by high… CONTINUE READING