Two-dimensional fluorescence spectroscopy: a novel approach for controlling fed-batch cultivations.

@article{Hantelmann2006TwodimensionalFS,
  title={Two-dimensional fluorescence spectroscopy: a novel approach for controlling fed-batch cultivations.},
  author={Kirsten Hantelmann and M Kollecker and Dirk H{\"u}ll and Bernd Hitzmann and Thomas Scheper},
  journal={Journal of biotechnology},
  year={2006},
  volume={121 3},
  pages={410-7}
}
In industrial fed-batch cultivations it is often necessary to control substrate concentrations at a low level to prevent the production of overflow metabolites and thus optimize the biomass yield. A new method for on-line monitoring and fed-batch control based on fluorescence measurements has been developed. Via instantaneous in situ measurements and multivariate data analysis a chemometric model has been established, which enables the rapid detection of ethanol production at aerobic… CONTINUE READING