Two-dimensional electrophoresis of proteins: a simple procedure for casting of narrow-bore, first dimension (isoelectric focusing) polyacrylamide tube gels.


A convenient and rapid method for casting of narrow-bore (1.5-2.5 mm, i.d.) first dimension isoelectric focusing gel tubes is described. The procedure utilizes a peristaltic pump and a 2-inch, 23 gauge blunt needle for dispensing the gel into fixed tubes on a 1.1 degree incline. The method is simple to perform, precludes the formation of air bubbles and… (More)