Two-dimensional electrophoresis of membrane proteins.

  title={Two-dimensional electrophoresis of membrane proteins.},
  author={Ralf J Braun and Norbert Kinkl and M. Beer and Marius Ueffing},
  journal={Analytical and bioanalytical chemistry},
  volume={389 4},
One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conventional two-dimensional gel electrophoresis (2-DE), a powerful electrophoretic method for the separation of complex protein samples that applies isoelectric focusing (IEF) in the first dimension and sodium dodecyl sulfate polyacrylamide gel… CONTINUE READING


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