Two-dimensional electrophoresis of membrane proteins.

@article{Braun2007TwodimensionalEO,
  title={Two-dimensional electrophoresis of membrane proteins.},
  author={Ralf J Braun and Norbert Kinkl and M. Beer and Marius Ueffing},
  journal={Analytical and bioanalytical chemistry},
  year={2007},
  volume={389 4},
  pages={1033-45}
}
One third of all genes of various organisms encode membrane proteins, emphasizing their crucial cellular role. However, due to their high hydrophobicity, membrane proteins demonstrate low solubility and a high tendency for aggregation. Indeed, conventional two-dimensional gel electrophoresis (2-DE), a powerful electrophoretic method for the separation of complex protein samples that applies isoelectric focusing (IEF) in the first dimension and sodium dodecyl sulfate polyacrylamide gel… CONTINUE READING

Citations

Publications citing this paper.

References

Publications referenced by this paper.
Showing 1-3 of 3 references

Part IV: internal organization of the cell

  • B Alberts, D Bray, J Lewis
  • Molecular biology of the cell
  • 2002
Highly Influential
5 Excerpts

Völkl A (2002) Electrophoresis

  • H Mohr
  • 2002
1 Excerpt

Similar Papers

Loading similar papers…