Two-dimensional NMR as a probe of structural similarity applied to mutants of cytochrome c.

@article{Pielak1988TwodimensionalNA,
  title={Two-dimensional NMR as a probe of structural similarity applied to mutants of cytochrome c.},
  author={Gary J Pielak and Robert A. Atkinson and Jonathan Boyd and Robert J Williams},
  journal={European journal of biochemistry},
  year={1988},
  volume={177 1},
  pages={179-85}
}
Using site-directed mutagenesis, it is possible to prepare many mutants of a protein in a short time, and to uncover differences in function. To understand the changes in function, it is essential to understand the effect(s) of the mutation in terms of structural and dynamic changes. It is particularly important to establish a rapid method for comparing the structure of the mutants with that of the wild-type protein. We propose that a combination of overlayed and difference two-dimensional NOE… CONTINUE READING

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