Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes.

@article{Nakagawa2013TwoDF,
  title={Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes.},
  author={Yosuke Nakagawa and Sou Nakamura and Masahiro Nakajima and Hiroshi Endo and Takeaki Dohda and Naoya Takayama and Hiromitsu Nakauchi and Fumihito Arai and Toshio Fukuda and Koji Eto},
  journal={Experimental hematology},
  year={2013},
  volume={41 8},
  pages={742-8}
}
Induced pluripotent stem cell (iPSC) technology enables us to investigate various potential iPSC-based therapies. Although the safety of iPSC derivation has not been completely validated, anucleate cells, such as platelets or erythrocytes, derived from iPSCs are promising targets. However, the efficiency of in vitro platelet generation from megakaryocytes (MKs) under static culture conditions is lower than is seen in vivo. In this study, we demonstrate the proof of concept by a two-dimensional… CONTINUE READING