Two different monoclonal antibodies to alpha-tubulin inhibit the bending of reactivated sea urchin spermatozoa.


Two monoclonal antibodies reactive for alpha-tubulin but not for beta-tubulin have been prepared, characterized in terms of their relative binding to tubulins from different sources by a solid-phase binding assay, immunoautoradiography, and indirect immunofluorescence, and utilized to study flagellar motility. Our results demonstrate that alpha-tubulins… (More)