Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant

@article{Ko2003TwoCF,
  title={Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant},
  author={Tae-Seok Ko and Sangman Lee and Sergei F. Krasnyanski and Schuyler S. Korban},
  journal={Theoretical and Applied Genetics},
  year={2003},
  volume={107},
  pages={439-447}
}
An efficient transformation system was developed for multiple soybean [Glycine max (L.) Merrill.] cultivars using Agrobacterium-mediated gene transfer. A significantly high number of hygromycin-resistant somatic embryos (SEs) was obtained when immature zygotic cotyledons were inoculated with Agrobacterium tumefaciens strain KYRT1 and when the abaxial side… CONTINUE READING