Two consecutive microtubule-based epithelial seaming events mediate dorsal closure in the scuttle fly Megaselia abdita

  title={Two consecutive microtubule-based epithelial seaming events mediate dorsal closure in the scuttle fly Megaselia abdita},
  author={Juan J. Fraire-Zamora and Johannes Jaeger and J{\'e}r{\^o}me Solon},
Evolution of morphogenesis is generally associated with changes in genetic regulation. Here we report evidence indicating that dorsal closure, a conserved morphogenetic process in dipterans, evolved as the consequence of rearrangements in epithelial organization rather than signaling regulation. In Drosophila melanogaster, dorsal closure consists of a two-tissue system where the contraction of extraembryonic amnioserosa and a JNK/Dpp-dependent epidermal actomyosin cable result in microtubule… 

How two extraembryonic epithelia became one: serosa and amnion features and functions of Drosophila's amnioserosa

It is concluded that the amnioserosa combines cells, genetic pathway components and functions that were previously associated either with serosa development or amnion development, raising the question of whether merging tissue-specific gene networks is a common evolutionary process.



Dynamic analysis of filopodial interactions during the zippering phase of Drosophila dorsal closure

A fly is generated in which RFP-Moesin and GFP- Moesin are expressed in mutually exclusive stripes within each segment using the engrailed and patched promoters, and mutually exclusive interactions between the filopodia of engrailing and patched cells are observed.

High plasticity in epithelial morphogenesis during insect dorsal closure

This work examines DC in the red flour beetle, Tribolium castaneum, providing the first detailed, functional analysis of DC in an insect with complete EE tissues (distinct amnion and serosa), and finds that the EE domain is particularly plastic in morphogenetic behavior and tissue structure.

Multiple Forces Contribute to Cell Sheet Morphogenesis for Dorsal Closure in Drosophila

The Drosophila embryo is established as an excellent system for the investigation of wound healing because it rapidly and reproducibly heals from both mechanical and ultraviolet laser wounds, even those delivered repeatedly.

Planar polarity and actin dynamics in the epidermis of Drosophila

It is shown that actin dynamics at the leading edge is preceded by a planar polarization of the dorsal-most epidermal cells associated with a reorganization of the cytoskeleton, and that Wingless (Wg) signalling and Jun amino-terminal kinase (JNK) signalling have overlapping but different roles in these events.

Quantitative analysis of cytoskeletal reorganization during epithelial tissue sealing by large-volume electron tomography

This work reconstructed a model closure event, dorsal closure in fly embryos, by large-volume correlative electron tomography, and presents a comprehensive, quantitative analysis of the cytoskeletal reorganization, enabling separated epidermal cells to seal the epithelium.

Dynamic analysis of dorsal closure in Drosophila: from genetics to cell biology.

Coupling of Jun amino-terminal kinase and Decapentaplegic signaling pathways in Drosophila morphogenesis.

Dorsal closure couples JNK and dpp signaling pathways, a situation that may be conserved in vertebrate development and suggest that the leading edge is the source of a JNK autocrine signal.