Two conformationally vicinal thiols at the active site of Leishmania donovani adenosine kinase.

@article{Bagui1996TwoCV,
  title={Two conformationally vicinal thiols at the active site of Leishmania donovani adenosine kinase.},
  author={T K Bagui and Monidipa Ghosh and Alok K Datta},
  journal={The Biochemical journal},
  year={1996},
  volume={316 ( Pt 2)},
  pages={439-45}
}
Inactivation of adenosine kinase (Adk) from Leishmania donovani correlates with the modification of two conformationally vicinal cysteine residues. In contrast, Adk from hamster liver, despite being sensitive to monothiol-blocking reagents, was insensitive to dithiol modifiers. Inactivation kinetics and substrate-protection studies along with double-modification experiments successively with N-ethylmaleimide in the presence of Ado and sodium m-arsenite-2,3-dimercaptopropanol or… CONTINUE READING

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