Two components of cell-bound isopullulanase from Aspergillus niger ATCC 9642--their purification and enzymatic properties.


Cell-bound isopullulanase (pullulan 4-glucanohydrolase: EC, IPU) from Aspergillus niger ATCC9642 [Y. Sakano et al., Denpun Kagaku, 37, 39-41 (1990)] was separated into two active components, IPU F1 (pI = 5.0) and IPU F2 (pI = 4.9), using a Mono-P HR 5/20 column. The substrate specificity on pullulan and panose, specific activity, optimum pH, pH… (More)


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