Two-color fluorescence analysis of individual virions determines the distribution of the copy number of proteins in herpes simplex virus particles.

@article{Clarke2007TwocolorFA,
  title={Two-color fluorescence analysis of individual virions determines the distribution of the copy number of proteins in herpes simplex virus particles.},
  author={Richard W. Clarke and Nilah Monnier and Haitao Li and Dejian Zhou and Helena Browne and David Klenerman},
  journal={Biophysical journal},
  year={2007},
  volume={93 4},
  pages={1329-37}
}
We present a single virion method to determine absolute distributions of copy number in the protein composition of viruses and apply it to herpes simplex virus type 1. Using two-color coincidence fluorescence spectroscopy, we determine the virion-to-virion variability in copy numbers of fluorescently labeled tegument and envelope proteins relative to a capsid protein by analyzing fluorescence intensity ratios for ensembles of individual dual-labeled virions and fitting the resulting histogram… CONTINUE READING

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