Two chicken B cell lines resistant to ouabain for the production of chicken monoclonal antibodies.

@article{Nishinaka1996TwoCB,
  title={Two chicken B cell lines resistant to ouabain for the production of chicken monoclonal antibodies.},
  author={S Nishinaka and Hitoshi Akiba and Masayuki Nakamura and Kumiko Suzuki and Tadaki Suzuki and K Tsubokura and Hiroyuki Horiuchi and Shuichi Furusawa and Haruo Matsuda},
  journal={The Journal of veterinary medical science},
  year={1996},
  volume={58 11},
  pages={1053-6}
}
Two chicken B cell lines, termed MuH1 and MuH4, which are resistant to ouabain, were established as fusion partners for production of chicken monoclonal antibody from the parental cell lines R27H1 and R27H4 which were deficient in thymidine kinase activity. The MuH1 synthesized, but did not secrete mu chain, whereas the MuH4 secreted non-specific IgM. Fusions of the MuH1 and MuH4 with spleen cells from chickens immunized with human IgG resulted in successful preparations of hybridomas secreting… CONTINUE READING