Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose.

@article{Schini1988TwocellBT,
  title={Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose.},
  author={S A Schini and Barry D. Bavister},
  journal={Biology of reproduction},
  year={1988},
  volume={39 5},
  pages={1183-92}
}
The failure of hamster 2-cell embryos to develop in vitro (2-cell block) was examined with experiments in which concentrations of glucose and phosphate in the culture medium were varied. Embryos were cultured in a protein-free modified Tyrode's solution that normally contains 5.0 mM glucose and 0.35 mM sodium dihydrogen phosphate. In the presence of 0.35 mM phosphate but without glucose, 23% of 2-cell embryos reached the 4-cell stage or further after culture for 1 day and 27% after 2 days… CONTINUE READING
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