Two cat expression vectors for cloning and generation of 3'- and 5'-deletion mutants.

Abstract

The construction of two versatile cat vectors, pGK0CAT and pGKA10CAT, is reported. These vectors possess multiple cloning sites derived from the Bluescript pKS(+) plasmid that allow the cloning of diverse DNA fragments. From a single cloned insert, i.e., a putative promoter element, one can use the exonuclease III (ExoIII) and S1 or mung-bean nuclease… (More)

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