Two cases of acanthamoeba keratitis diagnosed only by real-time polymerase chain reaction.

Abstract

PURPOSE To report 2 cases of Acanthamoeba keratitis whose causative pathogen was detected only by real-time polymerase chain reaction (PCR). METHODS Histological examinations of corneal scrapings were stained with Fungiflora Y. Corneal scrapings were also cultured on nonnutrient agar. Real-time PCR analyses of corneal scrapings were also performed… (More)
DOI: 10.1097/ICO.0b013e3181a39020

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