Two bacterial collagenolytic serine proteases have different topological specificities.

@article{Uesugi2008TwoBC,
  title={Two bacterial collagenolytic serine proteases have different topological specificities.},
  author={Yoshiko Uesugi and Jiro Arima and Hirokazu Usuki and Masaki Iwabuchi and Tadashi Hatanaka},
  journal={Biochimica et biophysica acta},
  year={2008},
  volume={1784 4},
  pages={716-26}
}
From among 2000 soil isolates, we purified a secreted serine protease from Streptomyces omiyaensis (SOT), which has extremely high gelatinolytic activity. Using sequence analysis, the primary structure of SOT showed 77% identity with that of S. griseus trypsin (SGT). We constructed recombinants SOT and SGT using S. lividans. They indicated similar properties on optimum pH and temperature, thermostability, and substrate preference using fluorescence energy transfer combinatorial libraries. SOT… CONTINUE READING