Two alternative forms of cDNA encoding CD34.

  title={Two alternative forms of cDNA encoding CD34.},
  author={Yukio Nakamura and H Komano and Hiromitsu Nakauchi},
  journal={Experimental hematology},
  volume={21 2},
By expression cloning using FACS, we have isolated cDNA clones encoding human CD34 from a megakaryoblastoid cell line. The predicted amino acid sequence of CD34 revealed the type I transmembrane protein consisted of a leader peptide (31 residues), an extracellular domain (258 residues), a transmembrane domain (23 residues) and a cytoplasmic domain of 73 residues. In addition, a second form of cDNA that has 194 bp insertion in the cytoplasmic region was isolated. The analysis of genomic DNA… CONTINUE READING


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