Two adjacent nuclear genes are required for functional complementation of a chloroplast trans-splicing mutant from Chlamydomonas reinhardtii.

@article{Balczun2005TwoAN,
  title={Two adjacent nuclear genes are required for functional complementation of a chloroplast trans-splicing mutant from Chlamydomonas reinhardtii.},
  author={Carsten Balczun and Astrid Bunse and Daniela Hahn and Pierre Bennoun and J{\"o}rg Nickelsen and Ulrich K{\"u}ck},
  journal={The Plant journal : for cell and molecular biology},
  year={2005},
  volume={43 5},
  pages={636-48}
}
The chloroplast tscA gene from Chlamydomonas reinhardtii encodes a co-factor RNA that is involved in trans-splicing of exons 1 and 2 of the psaA mRNA encoding a core polypeptide of photosystem I. Here we provide molecular and genetic characterization of the trans-splicing mutant TR72, which is defective in the 3'-end processing of the tscA RNA and consequently defective in splicing exons 1 and 2 of the psaA mRNA. Using genomic complementation, two adjacent nuclear genes were identified, Rat1… CONTINUE READING