Two Types of Phytases (Histidine Acid Phytase and β-Propeller Phytase) in Serratia sp. TN49 from the Gut of Batocera horsfieldi (Coleoptera) Larvae

  title={Two Types of Phytases (Histidine Acid Phytase and $\beta$-Propeller Phytase) in Serratia sp. TN49 from the Gut of Batocera horsfieldi (Coleoptera) Larvae},
  author={Rui Zhang and Peilong Yang and Huo-qing Huang and Pengjun Shi and Tie-zheng Yuan and Bin Yao},
  journal={Current Microbiology},
Microbial phytases play a major role in the mineralization of organic phosphorous, especially in symbiotic plants and animals. In this study, we identified two types of phytases in Serratia sp. TN49 that was harbored in the gut of Batocera horsfieldi (Coleoptera) larvae. The two phytases, an acidic histidine acid phosphatase (PhyH49) and an alkaline β-propeller phytase (PhyB49), shared low identities with known phytases (61% at most). PhyH49 and PhyB49 produced in Escherichia coli exhibited… 
In silico structural, functional and phylogenetic analysis of Klebsiella phytases
A strong correlation was found to be pertinent from phylogenetic studies of proteins with their cDNA among both species and strain level and to meet the demand of agricultural and industrial production of bacterial phytases particularly for agricultural farming.
Phytase Production from a Novel Klebsiella sp. on Wheat Bran for Animal Feed Digestion
The present findings will be very useful for the development of a bioprocess of the enzyme for its large-scale production at the pilot and finally at the commercial level.
Cloning and characterization of the first actinomycete β‐propeller phytase from Streptomyces sp. US42
Considering its biochemical properties, PHY US42 could be used as feed additive in combination with an acid phytase for monogastric animals.
Screening and Characterization of Phytases from Bacteria Isolated from Chilean Hydrothermal Environments
The characterization of phytase from two selected isolates revealed that both isolates produce phytases with a pH optimum at 5.0 and the temperature optimum for phytate dephosphorylation was determined to be 60 and 50 °C for the phytased from the isolates 9B and 15C, respectively.
Phytases from Enterobacter and Serratia species with desirable characteristics for food and feed applications
The novel phytases from these isolates have multiple characteristics of high thermostability and good phytase activity at desirable range of pH and temperature for their efficient use in food and feed to facilitate hydrolysis of phytate-metal ion complex and in turn, increased bioavailability of important metal ions to monogastric animals.
Crystallization and X-ray diffraction analysis of native and selenomethionine-substituted PhyH-DI from Bacillus sp. HJB17.
  • Fang LuBei Zhang B. Yao
  • Chemistry
    Acta crystallographica. Section F, Structural biology communications
  • 2017
Phytases are phosphatases that hydrolyze phytates to less phosphorylated myo-inositol derivatives and inorganic phosphate. β-Propeller phytases, which are very diverse phytases with improved
Fingerprint motifs of phytases
PAPhys is the major phytases, and HAPhys are the minor, and other phytase groups are not found in planta, but no fingerprint motifs are found by MAST for ALPhys.


A novel beta-propeller phytase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive
R-PhyP had higher relative activity at 25°C, and hydrolyzed phytate from soybean with greater efficacy at neutral pH, and might be a good candidate for an aquatic feed additive in the aquaculture industry.
Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiella sp. ASR1
The phytase protein was purified and characterised as a 42 kDa protein accepting phytate, NADP and sugar phosphates as substrates, and the corresponding gene (phyK) was cloned from chromosomal DNA using a combined approach of protein and genome analysis and expressed in Escherichia coli.
Diversity of Beta-Propeller Phytase Genes in the Intestinal Contents of Grass Carp Provides Insight into the Release of Major Phosphorus from Phytate in Nature
Beta-propeller phytase (BPP) gene fragments were readily amplified from the intestinal contents of grass carp directly or from phytate-degrading isolates from the same source, confirming the widespread occurrence of BPP in aquatic communities and the first study to experimentally estimate BPP diversity in situ.
Beta-propeller phytases in the aquatic environment
The presence of multiple sequences with a high similarity to phyS in aquatic environmental samples and the widespread occurrence of the Shewanella species in nature suggest that the β-propeller phytase family is the major class of phytases in the aquatic environment, and that it may play an important role in the recycling of phosphorus.
Distribution and diversity of phytate-mineralizing bacteria
Analysis of the genetic contexts of β-propeller phytase genes showed that these two genes are functionally linked and thus may play an important role in the cycles of phosphorus and iron.
A novel phytase with preferable characteristics from Yersinia intermedia.
Diversity, abundance and characterization of ruminal cysteine phytases suggest their important role in phytate degradation.
This study provides valuable information about the diversity, abundance and enzymatic properties of the ruminal cysteine phytases and emphasizes the important role(s) of these cysteines probably in the terrestrial cycle of phosphorus.
Biochemical properties and substrate specificities of alkaline and histidine acid phytases
This review describes recent findings that present novel viewpoints concerning the molecular basis of phytase classification and exhibits strict substrate specificity for the calcium–phytate complex and produces myo-inositol trisphosphate as the final product.
Purification and characterization of two phytases from Escherichia coli.
The chemical and kinetic properties of the purified phytase P2 points to an identity with an enzyme described by Dassa et al. (1982) as a pH 2.5 acid phosphatase; consequently, this enzyme is a 6-phytase.
Bacteria Associated with the Guts of Two Wood-Boring Beetles: Anoplophora glabripennis and Saperda vestita (Cerambycidae)
Analyzing the bacterial commensal communities of the exotic Asian longhorned beetle, Anoplophora glabripennis, and the native linden borer by randomly sequencing 16S rRNA gene fragments from bacterial DNA extracted directly from the gut of larvae may lead to new strategies to reduce the effects of these pest species.