Two-Photon Microscopy of Cells and Tissue

  title={Two-Photon Microscopy of Cells and Tissue},
  author={Brian D. O’Rourke},
Two-photon excitation fluorescence imaging provides thin optical sections from deep within thick, scattering specimens by way of restricting fluorophore excitation (and thus emission) to the focal plane of the microscope. Spatial confinement of two-photon excitation gives rise to several advantages over single-photon confocal microscopy. First, penetration depth of the excitation beam is increased. Second, because out-of-focus fluorescence is never generated, no pinhole is necessary in the… CONTINUE READING