• Corpus ID: 10806984

Two-Photon Fluorescence Microscopy : Basic Principles , Advantages and Risks

@inproceedings{Mulligan2007TwoPhotonFM,
  title={Two-Photon Fluorescence Microscopy : Basic Principles , Advantages and Risks},
  author={Sean J. Mulligan and Brian A. MacVicar},
  year={2007}
}
The application of two-photon excitation to fluorescence microscopy has become a powerful tool for studying biological function in live tissue and offers many advantages over conventional imaging techniques. Neuroscientists in particular have used this technology to image physiological functioning in microscopic and subcellular neural compartments. Neurons can be imaged deep within highly light scattering tissue with unparalleled spatial resolution and dramatically reduced photodynamic tissue… 

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