Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination

  title={Two Mutually Exclusive Local Chromatin States Drive Efficient V(D)J Recombination},
  author={Daniel J. Bolland and Hashem Koohy and Andrew L. Wood and Louise S. Matheson and Felix Krueger and Michael J.T. Stubbington and Amanda Baizan-Edge and Peter Chovanec and Bryony A. Stubbs and Kristina Tabbada and Simon R. Andrews and Mikhail Spivakov and Anne E Corcoran},
  booktitle={Cell reports},
Variable (V), diversity (D), and joining (J) (V(D)J) recombination is the first determinant of antigen receptor diversity. Understanding how recombination is regulated requires a comprehensive, unbiased readout of V gene usage. We have developed VDJ sequencing (VDJ-seq), a DNA-based next-generation-sequencing technique that quantitatively profiles recombination products. We reveal a 200-fold range of recombination efficiency among recombining V genes in the primary mouse Igh repertoire. We used… CONTINUE READING
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