Two‐photon image correlation spectroscopy and image cross‐correlation spectroscopy

@article{Wiseman2000TwophotonIC,
  title={Two‐photon image correlation spectroscopy and image cross‐correlation spectroscopy},
  author={Paul W. Wiseman and Jeffrey Squier and Mark Ellisman and Kent R. Wilson},
  journal={Journal of Microscopy},
  year={2000},
  volume={200}
}
We introduce two‐photon image correlation spectroscopy (ICS) using a video rate capable multiphoton microscope. We demonstrate how video rate two‐photon microscopic imaging and image correlation analysis may be combined to measure molecular transport properties over ranges typical of biomolecules in membrane environments. Using two‐photon ICS, we measured diffusion coefficients as large as 10−8 cm2 s−1 that matched theoretical predictions for samples of fluorescent microspheres suspended in… 
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TLDR
Novel extensions to ICS are developed that probe spatial correlations in previously inaccessible temporal windows and are used to determine for the first time spatially resolved diffusions of paxillin-EGFP stably expressed in CHOK1 cells.
Spatially resolved fluorescence correlation spectroscopy using a spinning disk confocal microscope.
We develop an extension of fluorescence correlation spectroscopy (FCS) using a spinning disk confocal microscope. This approach can spatially map diffusion coefficients or flow velocities at up to…
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This study investigates methods to filter out immobile or slow moving background structures and their impact on RICS results, and the capacity of RICS analysis to separate two species is studied.
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