Two‐photon image correlation spectroscopy and image cross‐correlation spectroscopy

@article{Wiseman2000TwophotonIC,
  title={Two‐photon image correlation spectroscopy and image cross‐correlation spectroscopy},
  author={Paul W. Wiseman and Jeffrey Squier and Mark Ellisman and Kent R. Wilson},
  journal={Journal of Microscopy},
  year={2000},
  volume={200}
}
We introduce two‐photon image correlation spectroscopy (ICS) using a video rate capable multiphoton microscope. We demonstrate how video rate two‐photon microscopic imaging and image correlation analysis may be combined to measure molecular transport properties over ranges typical of biomolecules in membrane environments. Using two‐photon ICS, we measured diffusion coefficients as large as 10−8 cm2 s−1 that matched theoretical predictions for samples of fluorescent microspheres suspended in… 
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