Twitch or swim: towards the understanding of prokaryotic motion based on the type IV pilus blueprint

@article{Daum2018TwitchOS,
  title={Twitch or swim: towards the understanding of prokaryotic motion based on the type IV pilus blueprint},
  author={Bertram Daum and Vicki A. M. Gold},
  journal={Biological Chemistry},
  year={2018},
  volume={399},
  pages={799 - 808}
}
Abstract Bacteria and archaea are evolutionarily distinct prokaryotes that diverged from a common ancestor billions of years ago. However, both bacteria and archaea assemble long, helical protein filaments on their surface through a machinery that is conserved at its core. In both domains of life, the filaments are required for a diverse array of important cellular processes including cell motility, adhesion, communication and biofilm formation. In this review, we highlight the recent… Expand
9 Citations
A new twist on bacterial motility – two distinct type IV pili revealed by cryoEM
TLDR
The structures answer key unresolved questions regarding the molecular architecture of type IV pili and identify a new type of pilin, and delineate the roles of the two filaments in promoting twitching and natural transformation. Expand
A comprehensive history of motility and Archaellation in Archaea
TLDR
A historical overview on archaella and motility research in Archaea is provided, beginning with the first simple observations of motile extreme halophilic archaea a century ago up to state-of-the-art cryo-tomography of the archaellum motor complex and filament observed today. Expand
The structures of two archaeal type IV pili illuminate evolutionary relationships
TLDR
The authors present the cryo-EM structures of two archaeal T4P from Pyrobaculum arsenaticum and Saccharolobus solfataricus and discuss evolutionary relationships between bacterial T 4P, Archaeal type IV pili, andArchaeal flagellar filaments. Expand
Molecular and Functional Analysis of the Type IV Pilus Gene Cluster in Streptococcus sanguinis SK36
TLDR
The results demonstrate that Tfp of SK36 are important for host cell adherence, but not for motility, and that expression of the pil cluster is subject to complex regulation. Expand
Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium
TLDR
Cryo-electron microscopy and mass spectrometry are used to show that the bacterium Thermus thermophilus produces two forms of type IV pilus (‘wide’ and ‘narrow’), differing in structure and protein composition. Expand
Scratching the Surface: Bacterial Cell Envelopes at the Nanoscale
TLDR
Recent progress in the use of AFM imaging for understanding the architecture and dynamics of the bacterial envelope is discussed, and the power of the technique to observe isolated membranes and live cells at (sub)nanometer resolution and under physiological conditions and to track in vitro structural dynamics in response to growth or to drugs is surveyed. Expand
Methods for Studying Swarming and Swimming Motility.
TLDR
Two agar-based assays for studying both swimming and swarming behavior, and considerations that affect the outcome are described here. Expand
Quantification of microaerobic growth of Geobacter sulfurreducens
TLDR
Investigation of microaerobic growth of G. sulfurreducens revealed that cell growth with oxygen is possible to the same extent as with fumarate if the maximum specific oxygen uptake rate (sOUR) of 95 mgO2 gCDW -1 h-1 is not surpassed, and transcriptome analysis suggests a menaquinol oxidase to be the enzyme responsible for oxygen reduction. Expand
Quantification of microaerobic growth of Geobacter sulfurreducens
TLDR
Investigation of microaerobic growth of G. sulfurreducens revealed that cell growth with oxygen is possible to the same extent as with fumarate if the maximum specific oxygen uptake rate (sOUR) of 95 mgO2 gCDW-1 h-1 is not surpassed, and transcriptome analysis suggests a menaquinol oxidase to be the enzyme responsible for oxygen reduction. Expand

References

SHOWING 1-10 OF 76 REFERENCES
The Archaellum: An Update on the Unique Archaeal Motility Structure.
TLDR
New data support the notion that the archaellum is evolutionary and structurally unrelated to the flagellum, but instead is related to archaeal and bacterial type IV pili and emphasize that it is a motility structure unique to the Archaea. Expand
How Bacteria Use Type IV Pili Machinery on Surfaces.
TLDR
The bacterial type IV pilus (T4P) is a versatile molecular machine with a broad range of functions, and its functions are diverse, and include adhesion, motility, and horizontal gene transfer. Expand
Assembly, Functions and Evolution of Archaella, Flagella and Cilia
TLDR
The evolutionary origins, assembly and tactic motility of archaella, flagella and cilia are compared, reflecting a striking example of convergent evolution. Expand
Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis
Archaeal swimming motility is driven by archaella: rotary motors attached to long extracellular filaments. The structure of these motors, and particularly how they are anchored in the absence of aExpand
Type IV pili and twitching motility.
  • J. Mattick
  • Biology, Medicine
  • Annual review of microbiology
  • 2002
TLDR
Twitching motility is a flagella-independent form of bacterial translocation over moist surfaces that is important in host colonization by a wide range of plant and animal pathogens, as well as in the formation of biofilms and fruiting bodies. Expand
Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery
TLDR
Using combined modes of electron cryo-microscopy (cryoEM) and cryoEM, the structure of the Pyrococcus furiosus archaellum filament is solved and the architecture and organisation of its motor complex in situ is visualised, paving the way to a molecular understanding of archaeal swimming motion. Expand
Structural differences in the bacterial flagellar motor among bacterial species
TLDR
The current findings on the divergent structures of the bacterial flagellar motor suggest that the flageLLar motors have adapted to function in various environments where bacteria live and survive. Expand
Motility and adhesion through type IV pili in Gram-positive bacteria.
TLDR
The current state of knowledge of type IV pilus systems in Gram-positive bacterial species and discusses them in the broader context of eubacterial type IV pili are reviewed. Expand
Pseudomonas aeruginosa twitching motility: type IV pili in action.
  • L. Burrows
  • Biology, Medicine
  • Annual review of microbiology
  • 2012
TLDR
This review covers recent findings concerning the three different types of T4P found in P. aeruginosa (type IVa, type IVb, and Tad) and provides details about the modes of translocation mediated by T4aP, the architecture and function of the T4 aP assembly system, and the complex regulation of T 4aP biogenesis and function. Expand
Asymmetric distribution of type IV pili triggered by directional light in unicellular cyanobacteria
TLDR
The sequential process of T4P dynamics from stimulus to taxis at the single-cell level in a model cyanobacterium, which can recognize light direction is demonstrated, providing clues toward a general regulation mechanism of the type IV pili system. Expand
...
1
2
3
4
5
...