Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli.

@article{Kim2005TwinarginineTO,
  title={Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli.},
  author={J E Kim and Elizabeth A Fogarty and Franklin J. Lu and Hui Zhu and Geoffrey D Wheelock and Lee A Henderson and Matthew P DeLisa},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 12},
  pages={8451-9}
}
When eukaryotic proteins with multiple disulfide bonds are expressed at high levels in Escherichia coli, the efficiency of thiol oxidation and isomerization is typically not sufficient to yield soluble products with native structures. Even when such proteins are secreted into the oxidizing periplasm or expressed in the cytoplasm of cells carrying mutations in the major intracellular disulfide bond reduction systems (e.g., trxB gor mutants), correct folding can be problematic unless a folding… CONTINUE READING
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