Twin arginine translocase pathway and fast-folding lipoprotein biosynthesis in E. coli: interesting implications and applications.

@article{Shruthi2010TwinAT,
  title={Twin arginine translocase pathway and fast-folding lipoprotein biosynthesis in E. coli: interesting implications and applications.},
  author={Hamsanathan Shruthi and Prakash Anand and V. Srinivasa Murugan and Krishnan Sankaran},
  journal={Molecular bioSystems},
  year={2010},
  volume={6 6},
  pages={999-1007}
}
Bacterial lipoproteins, an important class of membrane proteins, are generally thought to be translocated in an unfolded state by the well-studied Sec machinery, whereas the role of TAT, meant for folded proteins, is hardly investigated. Using appropriately engineered fast-folding Enhanced Green Fluorescence Protein (EGFP), as a model, here we show that TAT is essential for not only translocating fast-folding lipoprotein but also its lipid modification. EGFP was lipid-modified and targeted to… CONTINUE READING