Tus-Ter-lock immuno-PCR assays for the sensitive detection of tropomyosin-specific IgE antibodies.

  title={Tus-Ter-lock immuno-PCR assays for the sensitive detection of tropomyosin-specific IgE antibodies.},
  author={Elecia B. Johnston and Sandip D. Kamath and Andreas Ludwig Lopata and Patrick M. Schaeffer},
  volume={6 4},
BACKGROUND The increasing prevalence of food allergies requires development of specific and sensitive tests capable of identifying the allergen responsible for the disease. The development of serologic tests that can detect specific IgE antibodies to allergenic proteins would, therefore, be highly received. RESULTS Here we present two new quantitative immuno-PCR assays for the sensitive detection of antibodies specific to the shrimp allergen tropomyosin. Both assays are based on the self… 

Figures from this paper

Determination of Specific Class E Immunoglobulins to Bet v 1 Birch Allergen by the Immuno-PCR Method

It was demonstrated thatiPCR sensitivity is comparable to ELISA sensitivity, and the titration curves of specific sera in iPCR demonstrate a linear dependence; this makes the developed method preferable for quantitative estimation of specific IgE in sera as compared with ELISA.

Comparative diagnostics of allergy using quantitative immuno-PCR and ELISA.

Higher sensitivity of qiPCR in identification of low titer IgE is a result of a higher linearity of q iPCR data, which is three- to ten-times higher sensitivity than ELISA in IgE estimation inLow titer sera.

Immuno-PCR, a new technique for the serodiagnosis of tuberculosis.

Immuno-PCR technology for detection of natural human antibodies against Lec disaccharide

The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described and the sensitivity of the assay for determination of low-affinity anti-LeC IgM has been found to be two orders of magnitude higher than the conventional ELISA with the same antigen.

Gold nanoparticle-based novel visual diagnostic method for the detection of specific IgE to test for food allergies

This multiplexed visual diagnostic system for specific IgE against different food allergens using plasmonic phenomena of the aggregated gold nanoparticles has been a rapid, cheap, sensitive and high through put assay for the detection and determination of the severity of food allergies with a very low sample requirement.

Immuno-PCR: An ultrasensitive immunoassay for biomolecular detection.



Detection of multiple allergen-specific IgEs on microarrays by immunoassay with rolling circle amplification.

ImmunoRCA represents a novel approach for signal amplification of antibody-antigen recognition events on microarrays for allergen-specific IgE antibodies in serum and can detect IgE in a format using high-density microarray of anti-human IgE printed on glass slides by a pin-tool type microarraying robot.

Comparison of four in vitro assays for specific IgE detection

The data indicate that CAP, Allercoat and RAST are satisfactory techniques for specific IgE determination, either for inhalants or for food allergens; CAP, however, offers the highest sensitivity without loss of specificity.

Comparison of VIDAS Stallertest and Pharmacia CAP assays for detection of specific IgE antibodies in allergic children.

This study indicates that the VIDAS Stallertest and Pharmacia CAP assays are feasible and replicable for measuring allergen-specific IgE.

IgG-detection devices for the Tus-Ter-lock immuno-PCR diagnostic platform.

The superiority of the TT-lock Immuno-PCR platform for the quasi universal quantitative detection of antigens and mammalian IgG was demonstrated in terms of sensitivity over an analogous Protein G-Peroxidase ELISA.

Diagnosis of allergy by an in-vitro test for allergen antibodies.

Clinical evaluation of a new enzymo-assay for allergen-specific IgE.

In sera from patients selected to be either clearly allergic or not allergic (as defined by the concordant results of case history, skin prick test, and provocation test), Stallerzym proved to be as accurate and reliable as the Phadezym IgE RAST currently available.

ELISA and immuno-polymerase chain reaction assays for the sensitive detection of melioidosis.

Universal immuno-PCR for ultra-sensitive target protein detection.

This work has substituted the fusion protein with commercially available biotinylated secondary antibodies and free streptavidin, modified the sample dilution solution and reduced washing steps to a reasonable scale, resulting in a procedure that retains the sensitivity and specificity of the original Immuno-PCR, but can be universally applied without any Ig source or (sub)class restrictions.

Evaluation of a Novel Rapid Test System for the Detection of Allergic Sensitization to Timothy Grass Pollen against Established Laboratory Methods and Skin Prick Test

Visual- and scanner-based interpretation of the ALFA results revealed excellent agreement, and 100% sensitivity and specificity was found versus all other methods.

Development and validation of a third generation allergen-specific IgE assay on the continuous random access IMMULITE 2000 analyzer.

The analytical performance of a quantitative chemiluminescent enzyme immunoassay for sIgE using the continuous random access IMMULITE 2000 system demonstrated good precision and linearity over its measuring range.