Turnip yellow mosaic virus RNAs with anticodon loop substitutions that result in decreased valylation fail to replicate efficiently.

Single and multiple nucleotide substitutions have been introduced into the anticodon loop of the tRNA-like structure of turnip yellow mosaic virus (TYMV) genomic RNA. We studied the effects of these mutations on in vitro valylation and on replication in Chinese cabbage protoplasts and plants. Only those mutants capable of efficient and complete valylation… CONTINUE READING