Turnip mosaic virus VPg interacts with Arabidopsis thaliana eIF(iso)4E and inhibits in vitro translation.

@article{Miyoshi2008TurnipMV,
  title={Turnip mosaic virus VPg interacts with Arabidopsis thaliana eIF(iso)4E and inhibits in vitro translation.},
  author={Hiroshi Miyoshi and Hayato Okade and Shinji Muto and Noriko Suehiro and Hideki Nakashima and Koji Tomoo and Tomohide Natsuaki},
  journal={Biochimie},
  year={2008},
  volume={90 10},
  pages={
          1427-34
        }
}
The interaction between turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) and Arabidopsis thaliana eukaryotic initiation factor (iso)4E (eIF(iso)4E) was investigated to address the influence of potyviral VPg on host cellular translational initiation. Affinity chromatographic analysis showed that the region comprising amino acids 62-70 of VPg is important for the interaction with eIF(iso)4E. In vitro translation analysis showed that the addition of VPg significantly inhibited… Expand
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The results suggest that VPg-eIF(iso)4E interaction is a critical element for virus production. Expand
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Viral VPg can interfere with formation of a translational initiation complex on host plant cellular mRNA by sequestering eIF(iso)4E, which suggests that VPg may participate in viral translational initiated by functioning as an alternative cap-like structure. Expand
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The capacity of TuMV VPgPro and of Norwalk virus (NV) VPg to degrade RNA suggests that these proteins have a ribonucleolytic activity which may contribute to the host RNA translation shutoff associated with many virus infections. Expand
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The quantitative data presented here suggest a model where eIF4F·VPg interaction enhances cap-independent translation by increasing the affinity of eif4F for TEV RNA, the first evidence of direct participation of VPg in translation initiation. Expand
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TLDR
The yeast LexA interaction trap was used to screen a cDNA library from Arabidopsis thaliana in order to identify proteins that interact with the viral protein genome linked (VPg)-proteinase of turnip mosaic potyvirus and it was found that eIF(iso)4E plays an essential role in the initiation of the translation of capped mRNAs and its association with VPg would point to a role of the viralprotein in thetranslation of the virus. Expand
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The interaction between VPg of turnip mosaic virus and wheat germ eukaryotic translation initiation factors eIFiso4E and eIFiso4F (the complex of eIFiso4E and eIFiso4G) were measured and compared.Expand
Calicivirus translation initiation requires an interaction between VPg and eIF4E
TLDR
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The genome‐linked protein VPg of the Norwalk virus binds eIF3, suggesting its role in translation initiation complex recruitment
TLDR
The data suggest that VPg may play a role in initiating translation on calicivirus RNA through unique protein–protein interactions with the translation machinery. Expand
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