Turning of the receptor-binding domains opens up the murine leukaemia virus Env for membrane fusion

@article{Wu2008TurningOT,
  title={Turning of the receptor-binding domains opens up the murine leukaemia virus Env for membrane fusion},
  author={Shang-Rung Wu and Mathilda Sj{\"o}berg and Michael Wallin and Birgitta Lindqvist and Maria Ekstr{\"o}m and Hans Hebert and P. J. B. Koeck and Henrik Garoff},
  journal={The EMBO Journal},
  year={2008},
  volume={27},
  pages={2799 - 2808}
}
The activity of the membrane fusion protein Env of Moloney mouse leukaemia virus is controlled by isomerization of the disulphide that couples its transmembrane (TM) and surface (SU) subunits. We have arrested Env activation at a stage prior to isomerization by alkylating the active thiol in SU and compared the structure of isomerization-arrested Env with that of native Env. Env trimers of respective form were isolated from solubilized particles by sedimentation and their structures were… CONTINUE READING