Tuning the activity of Zn(II) complexes in DNA cleavage: clues for design of new efficient metallo-hydrolases.

Abstract

The hydrolytic ability toward plasmid DNA of a mononuclear and a binuclear Zn(II) complex with two macrocyclic ligands, containing respectively a phenanthroline (L1) and a dipyridine moiety (L2), was analyzed at different pH values and compared with their activity in bis( p-nitrophenyl)phosphate (BNPP) cleavage. Only the most nucleophilic species [ZnL1(OH)]+ and [Zn2L2(OH)2]2+, present in solution at alkaline pH values, are active in BNPP cleavage, and the dinuclear L2 complex is remarkably more active than the mononuclear L1 one. Circular dichroism and unwinding experiments show that both complexes interact with DNA in a nonintercalative mode. Experiments with supercoiled plasmid DNA show that both complexes can cleave DNA at neutral pH, where the L1 and L2 complexes display a similar reactivity. Conversely, the pH-dependence of their cleavage ability is remarkably different. The reactivity of the mononuclear complex, in fact, decreases with pH while that of the dinuclear one is enhanced at alkaline pH values. The efficiency of the two complexes in DNA cleavage at different pH values was elucidated by means of a quantum mechanics/molecular mechanics (QM/MM) study on the adducts between DNA and the different complexed species present in solution.

DOI: 10.1021/ic800085n

Cite this paper

@article{Bazzicalupi2008TuningTA, title={Tuning the activity of Zn(II) complexes in DNA cleavage: clues for design of new efficient metallo-hydrolases.}, author={Carla Bazzicalupi and Andrea Bencini and Claudia Bonaccini and Claudia De Giorgi and Paola Gratteri and Stefano Moro and Manlio Palumbo and Alessandro Simionato and Jacopo Sgrignani and Claudia Sissi and Barbara Valtancoli}, journal={Inorganic chemistry}, year={2008}, volume={47 12}, pages={5473-84} }