Tuning protein expression using synonymous codon libraries targeted to the 5' mRNA coding region.


In bacteria, the 5' mRNA coding region plays an important role in determining translation output. Here, we report synthetic sequences that when placed in the 5'-mRNA coding region, leading to recombinant proteins containing short N-terminal extensions, virtually abolish, enhance or produce intermediate expression levels of green fluorescent protein in… (More)
DOI: 10.1093/protein/gzq086