Tuning genetic control through promoter engineering.

@article{Alper2005TuningGC,
  title={Tuning genetic control through promoter engineering.},
  author={Hal S. Alper and Curt R Fischer and Elke Nevoigt and Gregory Stephanopoulos},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 36},
  pages={
          12678-83
        }
}
Gene function is typically evaluated by sampling the continuum of gene expression at only a few discrete points corresponding to gene knockout or overexpression. We argue that this characterization is incomplete and present a library of engineered promoters of varying strengths obtained through mutagenesis of a constitutive promoter. A multifaceted characterization of the library, especially at the single-cell level to ensure homogeneity, permitted quantitative assessment correlating the effect… CONTINUE READING

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