Tuning SpyTag-SpyCatcher mutant pairs toward orthogonal reactivity encryption.


Genetically encoded covalent peptide tagging technology, such as the SpyTag-SpyCatcher reaction, has emerged as a unique way to do chemistry with proteins. Herein, we report the reactivity engineering of SpyTag-SpyCatcher mutant pairs and show that distinct reactivity can be encrypted for the same reaction based on protein sequences of high similarity… (More)
DOI: 10.1039/c7sc02686b