Tunicamycin modulates binding of 125I-erythropoietin to Friend erythroleukemia cells.

Abstract

The effects of tunicamycin and neuraminidase treatment on the specific binding of 125I-erythropoietin to a murine erythroleukemia cell clone, B8, were investigated. Neuraminidase treatment of B8 cells did not affect the specific binding of erythropoietin, but tunicamycin treatment caused a 2.5 to 4-fold increase in the amount of 125I-erythropoietin binding. Scatchard analysis of the binding data showed that the increase in the amount of binding resulted from increased affinity of the receptor. These results suggest that N-linked sugars of the erythropoietin receptor protein are involved in the interaction of erythropoietin with the cell-surface receptors on B8 cells.

Cite this paper

@article{Fukamachi1989TunicamycinMB, title={Tunicamycin modulates binding of 125I-erythropoietin to Friend erythroleukemia cells.}, author={Hiromi Fukamachi and Akihiro Tojo and T Saito and Tadaichi Kitamura and Akio Urabe and Fumimaro Takaku}, journal={Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society}, year={1989}, volume={52 1}, pages={1-6} }