• Medicine
  • Published in Agents and actions 1993

Tumour necrosis factor alpha production by rat blood and its ex vivo pharmacological modulation.

@article{Foster1993TumourNF,
  title={Tumour necrosis factor alpha production by rat blood and its ex vivo pharmacological modulation.},
  author={Stephen James Foster and Lynn M. McCormick},
  journal={Agents and actions},
  year={1993},
  volume={39 Spec No},
  pages={
          C61-3
        }
}
Rat blood was investigated as a suitable test system for the discovery of inhibitors of tumour necrosis factor alpha (TNF alpha) biosynthesis. Lipopolysaccharide (LPS) caused a concentration- and time-dependent stimulation of TNF alpha production by heparinised rat blood with peak levels (1000-5000 U/ml; L929 bioassay) at 6 h. Bioactive material was neutralised with a polyclonal rabbit anti-murine TNF alpha antibody which cross-reacts with rat TNF alpha. Dexamethasone, pentoxifylline and… CONTINUE READING